A Live Cell Imaging System, composed of a wide field, inverted, fully motorized Olympus IX-81 microscope equipped with:
- Olympus IX8S1F-ZDC stage (inverted) for DIC and phase contrast imaging.
- a Hamamatsu CCD ORCA/AG cryocamera
- a fast excitation filter wheel
- Temperature and CO2 chamber
- hardware for FRET
- the CellR imaging software
- air conditioning unit
which allow fast multicolor 3D imaging, transmission microscopy (phase-contrast and Nomarski-DIC), in vivo time-lapse experiments, spectral unmixing of fluorescent dyes, FRET and 3D-dencovolution analysis.
