Laboratory of Molecular Neurobiology and Immunology

The Laboratory of Molecular Neurobiology and Immunology has been active in Research for more than three decades (former Head of Lab Prof. S. Tzartos) and consists of two research groups: a) The Diagnosis and Treatment of Neuroimmune Disorders with Dr. P. Zisimopoulou as principal investigator and b) Structural Neurobiology with Dr. M. Zouridakis as principal investigator.

The research focus of Zisimopoulou group is the diagnosis of autoimmune neurological diseases through the identification of novel antigens and the development of diagnostic assays. To the research interests belongs as well the characterization of the identified autoantigens to better understanding of the disease and specific treatment. More specifically, during the last years PI’s priorities were

The characterization of the autoantibodies in Myasthenia gravis. Myasthenia Gravis is an autoimmune neurological disorder, mainly caused by autoantibodies targeting the muscle nicotinic acetylcholine receptor (nAChR). The presentation of the disease is heterogenic and the antibody titer does not correlate with the disease severity between individuals. We investigate if some of the differences in clinical symptoms observed between patients with the same autoantibody titer is correlated to the presence of autoantibodies targeting different subunit of the receptor.

The involvement of neuronal nAChR in autoimmunity. The nAChRs are ligand gated ion channels that mediate transduction of chemoelectric signals throughout the nervous system by opening intrinsic ionic channels. They are widely distributed in the central and peripheral nervous systems. They have a role in directly stimulating not only pre- and postsynaptic neurons but also modulate blood flow. For many years the involvement of nAChRs has been investigated in several common neurological disorders such as schizophrenia, addiction, Alzheimer disease, ADHD and Parkinson disease.

The last few years we are trying to elucidate the role of different subtypes of nAChR in the degeneration of neurons associated with different neurodegenerative diseases. We immunized mice with the extracellular domains of several nAChR subunits β2, α4, α3, α7, α5 and subjected them to phenotypic and behavioral analysis.  In addition, we investigate the effect of the antibodies produced in the periphery.

The development of assays for the detection of autoantibodies against the acetylcholine receptors. Both neuronal nAChRs and muscarinic acetylcholine receptors (mAChRs) are distributed throughout the central and peripheral nervous system, where they play crucial roles during neurotransmission. Our hypothesis is that in sera from patients with autoimmune encephalitis and related syndromes, autoantibodies targeting these receptors exist. Therefore, new diagnostic assays (i.e. cell based assays-CBA, ELISA) suitable for the detection of the relevant autoantibodies will be developed and used for sera and CSF screening.

Finally, due to the pandemic, both research groups worked on the expression and characterization of COVID-19 RBD variants. The expressed proteins were used in crystallization trials and in assays for characterization of specific antibodies in sera of vaccinated individuals.

The research focus of Zouridakis group is the elucidation of the X-ray crystal structures of various proteins of pharmacological interest and of the structures of their complexes with ligands (i.e. chemical molecules, peptides) and/or antibodies that could serve as potential drugs for the treatment of related diseases. Moreover, of great interest is to decipher the functional mechanisms of the studied proteins through structure-guided mutagenesis and functional assays. Also, in collaboration with the Research group of Diagnosis and Treatment of Neuroimmune Disorders, which is the other group of the Laboratory of Molecular Neurobiology and Immunology, some of the recombinant proteins used for structural studies serve as antigens for the development of diagnostic assays.

Main research interests of the group:

Structural studies of ligand-gated ion channels. A major target for elucidation of its 3D-structure is the human neuronal nicotinic acetylcholine receptor (nAChR). Neuronal nAChRs regulate neuronal excitability and neurotransmitter release and exist in various pentameric subtypes formed by combinations between eleven subunits (α2-α7, α9, α10, β2-β4), each with distinct pharmacological and electrophysiological properties. They are responsible for nicotine addiction and are implicated in several neurological diseases and disorders (e.g. Alzheimer’s and Parkinson’s, autism, schizophrenia). Interestingly, neuronal nAChRs (e.g. α7, α9, α10) exist also in a variety of cells such as immune cells, breast and lung cells, keratinocytes, etc, and have been found to play important roles in inflammation and in various cancer types. Elucidation of the structure of neuronal nAChRs in their Apo and ligand-bound forms is needed for the design of subtype-specific drugs and for understanding their functional mechanisms. Also, of interest is the elucidation of the crystal structures of the human muscle nAChR (the autoantigen in myasthenia gravis disease) with novel neurotoxins and Fab domains of monoclonal antibodies.

Structural studies of the ACE2 receptor. Driven by the COVID-19 pandemic, the group has initiated efforts to elucidate the X-ray crystal structures of the human Angiotensin Converting Enzyme 2 receptor (ACE2), which is the established entry point of the SARS-CoV2 coronavirus to the cells, in its complexes with the receptor-binding domains (RBDs) of the original Wuhan strain and with the emerged variants. In addition, the group is interested in structural studies of ACE2 and ACE receptors with novel inhibitors in order to provide the structural basis for the design of novel ligands (inhibitors or activators) of the Renin-Angiotensin-Aldosterone System (RAAS) for the regulation of blood pressure and fluid balance. Moreover, the group provides the recombinantly expressed RBD and ACE2 proteins to the other group of the lab (Diagnosis and Treatment of Neuroimmune Disorders) for the development of Ab-detecting assays for vaccinated and recovered individuals.

Functional studies of receptors and enzymes. The group is interested in complementing its structural findings with functional studies. For nAChRs, the group studies their pharmacological properties and the effect of novel ligands with radio-ligand binding experiments and with two-electrode voltage clamp electrophysiology, while for the ACE2 enzyme the group studies the effect of potentially novel inhibitors (or activators) with classical kinetic studies. Also, of great interest is the discovery of autoantibodies against neuronal nAChRs in several diseases of the nervous system such as schizophrenia, bipolar disorders, epilepsy, etc, in collaboration with the other research group of the lab and with external collaborators.

Ongoing Research Support

Operational Program Competitiveness, Entrepreneurship and Innovation, European Union and Greek national funds, Biomarkers in autoimmune neurological dieseases, NeuroMarkers, MIS 5032815, 2018-2021

Operational Program Competitiveness, Entrepreneurship and Innovation, European Union and Greek national funds, Effectiveness of terrestrial and marine plant extracts for the prevention and treatment of parasite infections (Microcotyle spp, Myxosporea) and of myxobacteriosis in cultured Sea bream (Sparus aurata L.), AltMedSea-bream, MIS 5055881, 2020-2022

Stavros Niarchos Foundation, Development of innovative biological products and services for infectious and neurodegenerative diseases, 2017-2021

Hellenic Foundation for Research and Innovation (HFRI) Project, Structural and functional studies of nicotinic acetylcholine receptors, StruNic, code number 677, 2018-2022

Completed Research Support

Bilateral R&D Cooperation between Greece and Israel, Development of tools for Understanding and Diagnosis of Neuroimmune Disorders, NeuroID/3257, 2013-2015

EU Grants:

FP7. Neurotransmitter Cys-loop receptors: structure, function and disease. 2008-13

FP7. Fight-MG. Myasthenias, a group of immune mediated neurological diseases: from etiology to therapy. 2009-13

FP7-Regpot. Development of a center of excellence in Neurosignaling. 2010-13

Grants from other international sources

AFM. Characterization of autoantibody subgroups in myasthenia gravis. 2006-07

MDA USA. Antigen-specific therapeutic autoantibody depletion in myasthenia gravis. 2010-12

MDA USA. Diagnosis and characterization of LRP4-MG, a novel myasthenia gravis subtype. 2013-16

AFM. Diagnosis, prevalence and characterization of a novel myasthenia gravis subtype, LRP4-MG. 2013-15

Grants from Greek sources:

ARISTEIA, Myasthenia Gravis, 2012-15

2021

Topaloudi A, Zagoriti Z, Flint AC, Martinez MB, Yang Z, Tsetsos F, Christou YP, Lagoumintzis G, Yannaki E, Zamba-Papanicolaou E, Tzartos J, Tsekmekidou X, Kotsa K, Maltezos E, Papanas N, Papazoglou D, Passadakis P, Roumeliotis A, Roumeliotis S, Theodoridis M, Thodis E, Panagoutsos S, Yovos J, Stamatoyannopoulos J, Poulas K, Kleopa K, Tzartos S, Georgitsi M, Paschou P.J Myasthenia gravis genome-wide association study implicates AGRN as a risk locus. Med Genet. 2021 Aug 16:jmedgenet-2021-107953. doi: 10.1136/jmedgenet-2021-107953. Online ahead of print.PMID: 34400559

Chiniadis L, Giastas P, Bratsos I, Papakyriakou A. Insights into the Protein Ruthenation Mechanism by Antimetastatic Metallodrugs: High-Resolution X-ray Structures of the Adduct Formed between Hen Egg-White Lysozyme and NAMI-A at Various Time Points. Inorg Chem. 2021 Jul 19;60(14):10729-10737. doi: 10.1021/acs.inorgchem.1c01441. Epub 2021 Jul 1.PMID: 34197115

Muñoz U, Sebal C, Escudero E, Esiri M, Tzartos J, Sloan C, Sadaba MC. Main Role of Antibodies in Demyelination and Axonal Damage in Multiple Sclerosis. Cell Mol Neurobiol. 2021 Feb 24. doi: 10.1007/s10571-021-01059-6. Online ahead of print.PMID: 33625628

Koneczny I, Yilmaz V, Lazaridis K, Tzartos J, Lenz TL, Tzartos S, Tüzün E, Leypoldt F. Common Denominators in the Immunobiology of IgG4 Autoimmune Diseases: What Do Glomerulonephritis, Pemphigus Vulgaris, Myasthenia Gravis, Thrombotic Thrombocytopenic Purpura and Autoimmune Encephalitis Have in Common? Front Immunol. 2021 Jan 29;11:605214. doi: 10.3389/fimmu.2020.605214. eCollection 2020.PMID: 33584677 Free PMC article. Review.

Zouvelou V, Michail M, Belimezi M, Zisimopoulou P. Subunit specificity of the acetylcholine receptor antibodies in double seropositive myasthenia gravis. Muscle Nerve. 2021 Apr;63(4):E36-E37. doi: 10.1002/mus.27177. Epub 2021 Feb 5.PMID: 33471417 No abstract available.

2020

Lazaridis K, Tzartos SJ. Myasthenia Gravis: Autoantibody Specificities and Their Role in MG Management. Front Neurol. 2020 Nov 30;11:596981. doi: 10.3389/fneur.2020.596981. eCollection 2020.PMID: 33329350 Free PMC article. Review.

Yilmaz V, Ulusoy C, Hajtovic S, Turkoglu R, Kurtuncu M, Tzartos J, Lazaridis K, Tuzun E. Effects of Teriflunomide on B Cell Subsets in MuSK-Induced Experimental Autoimmune Myasthenia Gravis and Multiple Sclerosis. Immunol Invest. 2021 Aug;50(6):671-684. doi: 10.1080/08820139.2020.1785491. Epub 2020 Jun 29.PMID: 32597289 Clinical Trial.

Savaş M, Tzartos J, Küçükali Cİ, Dursun E, Karagiorgou K, Gezen-Ak D, Türkdoğan D, Papaconstantinou A, Başoğlu S, Hacıhafızoğlu N, Kutlubay B, Tzartos S, Tüzün E. Glial fibrillary acidic protein (GFAP)-antibody in children with focal seizures of undetermined cause.Acta Neurol Belg. 2021 Oct;121(5):1275-1280. doi: 10.1007/s13760-020-01361-y. Epub 2020 Apr 24.PMID: 32333263

Yetimler B, Tzartos J, Şengül B, Dursun E, Ulukan Ç, Karagiorgou K, Gezen-Ak D, Sezgin M, Papaconstantinou A, Tzartos S, Orhan EK, Ekizoğlu E, Küçükali Cİ, Baykan B, Tüzün E. Serum glial fibrillary acidic protein (GFAP)-antibody in idiopathic intracranial hypertension. Int J Neurosci. 2021 Aug;131(8):775-779. doi: 10.1080/00207454.2020.1758084. Epub 2020 Apr 28.PMID: 32303142

Chiniadis L, Bratsos I, Bethanis K, Karpusas M, Giastas P, Papakyriakou A. High-resolution crystal structures of a "half sandwich"-type Ru(II) coordination compound bound to hen egg-white lysozyme and proteinase K. J Biol Inorg Chem. 2020 Jun;25(4):635-645. doi: 10.1007/s00775-020-01786-z. Epub 2020 Apr 8.PMID: 32266561

Lazaridis K, Tzartos SJ. Autoantibody Specificities in Myasthenia Gravis; Implications for Improved Diagnostics and Therapeutics. Front Immunol. 2020 Feb 14;11:212. doi: 10.3389/fimmu.2020.00212. eCollection 2020.PMID: 32117321 Free PMC article. Review.

Tzartos JS, Karagiorgou K, Tzanetakos D, Breza M, Evangelopoulos ME, Pelidou SH, Bakirtzis C, Nikolaidis I, Koutsis G, Notas K, Chroni E, Markakis I, Grigoriadis NC, Anagnostouli M, Orologas A, Parisis D, Karapanayiotides T, Papadimitriou D, Kostadima V, Elloul J, Xidakis I, Maris T, Zisimopoulou P, Tzartos S, Kilidireas C. Deciphering anti-MOG IgG antibodies: Clinical and radiological spectrum, and comparison of antibody detection assays. J Neurol Sci. 2020 Mar 15;410:116673. doi: 10.1016/j.jns.2020.116673. Epub 2020 Jan 7.PMID: 31954354

2019

Lazaridis K, Baltatzidou V, Tektonidis N, Tzartos SJ. Antigen-specific immunoadsorption of MuSK autoantibodies as a treatment of MuSK-induced experimental autoimmune myasthenia gravis. J Neuroimmunol. 2020 Feb 15;339:577136. doi: 10.1016/j.jneuroim.2019.577136. Epub 2019 Dec 16.PMID: 31855721

Lebedev DS, Kryukova EV, Ivanov IA, Egorova NS, Timofeev ND, Spirova EN, Tufanova EY, Siniavin AE, Kudryavtsev DS, Kasheverov IE, Zouridakis M, Katsarava R, Zavradashvili N, Iagorshvili I, Tzartos SJ, Tsetlin VI. Oligoarginine Peptides, a New Family of Nicotinic Acetylcholine Receptor Inhibitors. Mol Pharmacol. 2019 Nov;96(5):664-673. doi: 10.1124/mol.119.117713. Epub 2019 Sep 6.PMID: 31492697

Zouridakis M, Papakyriakou A, Ivanov IA, Kasheverov IE, Tsetlin V, Tzartos S, Giastas P. Crystal Structure of the Monomeric Extracellular Domain of α9 Nicotinic Receptor Subunit in Complex With α-Conotoxin RgIA: Molecular Dynamics Insights Into RgIA Binding to α9α10 Nicotinic Receptors. Front Pharmacol. 2019 May 1;10:474. doi: 10.3389/fphar.2019.00474. eCollection 2019.PMID: 31118896 Free PMC article.

Gilhus NE, Tzartos S, Evoli A, Palace J, Burns TM, Verschuuren JJGM. Myasthenia gravis. Nat Rev Dis Primers. 2019 May 2;5(1):30. doi: 10.1038/s41572-019-0079-y.PMID: 31048702 Review.

Andreou A, Giastas P, Arnaouteli S, Tzanodaskalaki M, Tzartos SJ, Bethanis K, Bouriotis V, Eliopoulos EE. The putative polysaccharide deacetylase Ba0331: cloning, expression, crystallization and structure determination.Acta Crystallogr F Struct Biol Commun. 2019 Apr 1;75(Pt 4):312-320. doi: 10.1107/S2053230X19001766. Epub 2019 Apr 3.PMID: 30950833 Free PMC article.

Koneczny I, Rennspiess D, Marcuse F, Dankerlui N, Abdul Hamid M, Mané-Damas M, Maessen J, Van Schil P, Saxena A, Zisimopoulou P, Lazaridis K, Woodhall M, Karagiorgou K, Tzartos J, Tzartos S, De Baets MH, Molenaar PC, Marx A, Zur Hausen A, Losen M, Martinez-Martinez P. Characterization of the thymus in Lrp4 myasthenia gravis: Four cases. Autoimmun Rev. 2019 Jan;18(1):50-55. doi: 10.1016/j.autrev.2018.07.011. Epub 2018 Nov 8.PMID: 30414949

2018

Kryukova EV, Ivanov IA, Lebedev DS, Spirova EN, Egorova NS, Zouridakis M, Kasheverov IE, Tzartos SJ, Tsetlin VI. Orthosteric and/or Allosteric Binding of α-Conotoxins to Nicotinic Acetylcholine Receptors and Their Models. Mar Drugs. 2018 Nov 22;16(12):460. doi: 10.3390/md16120460.PMID: 30469507 Free PMC article.

Tüzün E, Gezen-Ak D, Tzartos J, Dursun E, Giriş M, Zisimopoulou P, Karagiorgou K, Yetimler B, Küçükali Cİ, İdrisoğlu HA. LRP4 antibody positive amyotrophic lateral sclerosis patients display neuropil-reactive IgG and enhanced serum complement levels. Immunol Lett. 2018 Nov;203:54-56. doi: 10.1016/j.imlet.2018.09.011. Epub 2018 Sep 15.PMID: 30227179 No abstract available.

Andreou A, Giastas P, Christoforides E, Eliopoulos EE. Structural and Evolutionary Insights within the Polysaccharide Deacetylase Gene Family of Bacillus anthracis and Bacillus cereus. Genes (Basel). 2018 Jul 31;9(8):386. doi: 10.3390/genes9080386.PMID: 30065210 Free PMC article.

Trakas N, Tzartos SJ. Immunostick ELISA for rapid and easy diagnosis of myasthenia gravis. J Immunol Methods. 2018 Sep;460:107-112. doi: 10.1016/j.jim.2018.06.016. Epub 2018 Jul 2.PMID: 30056940

Wonnacott S, Bermudez I, Millar NS, Tzartos SJ. Nicotinic acetylcholine receptors. Br J Pharmacol. 2018 Jun;175(11):1785-1788. doi: 10.1111/bph.14209.PMID: 29878346 Free PMC article.

Zagoriti Z, Lagoumintzis G, Perroni G, Papathanasiou G, Papadakis A, Ambrogi V, Mineo TC, Tzartos JS, Poulas K. Evidence for association of STAT4 and IL12RB2 variants with Myasthenia gravis susceptibility: What is the effect on gene expression in thymus? J Neuroimmunol. 2018 Jun 15;319:93-99. doi: 10.1016/j.jneuroim.2018.03.008. Epub 2018 Mar 17.PMID: 29576322

Giastas P, Andreou A, Papakyriakou A, Koutsioulis D, Balomenou S, Tzartos SJ, Bouriotis V, Eliopoulos EE. Structures of the Peptidoglycan N-Acetylglucosamine Deacetylase Bc1974 and Its Complexes with Zinc Metalloenzyme Inhibitors. Biochemistry. 2018 Feb 6;57(5):753-763. doi: 10.1021/acs.biochem.7b00919. Epub 2018 Jan 5.PMID: 29257674

Saxena A, Stevens J, Cetin H, Koneczny I, Webster R, Lazaridis K, Tzartos S, Vrolix K, Nogales-Gadea G, Machiels B, Molenaar PC, Damoiseaux J, De Baets MH, Simon-Keller K, Marx A, Vincent A, Losen M, Martinez-Martinez P. Characterization of an anti-fetal AChR monoclonal antibody isolated from a myasthenia gravis patient. Sci Rep. 2017 Oct 31;7(1):14426. doi: 10.1038/s41598-017-14350-8.PMID: 2908 9519 Free PMC article.

Giastas P, Zouridakis M, Tzartos SJ. Understanding structure-function relationships of the human neuronal acetylcholine receptor: insights from the first crystal structures of neuronal subunits. Br J Pharmacol. 2018 Jun;175(11):1880-1891. doi: 10.1111/bph.13838. Epub 2017 Jun 20.PMID: 28452148 Free PMC article. Review.

2017

Vulfius CA, Kasheverov IE, Kryukova EV, Spirova EN, Shelukhina IV, Starkov VG, Andreeva TV, Faure G, Zouridakis M, Tsetlin VI, Utkin YN. Pancreatic and snake venom presynaptically active phospholipases A2 inhibit nicotinic acetylcholine receptors. PLoS One. 2017 Oct 12;12(10):e0186206. doi: 10.1371/journal.pone.0186206. eCollection 2017.PMID: 29023569 Free PMC article.

Tzartos JS, Stergiou C, Daoussis D, Zisimopoulou P, Andonopoulos AP, Zolota V, Tzartos SJ. Antibodies to aquaporins are frequent in patients with primary Sjögren's syndrome. Rheumatology (Oxford). 2017 Dec 1;56(12):2114-2122. doi: 10.1093/rheumatology/kex328.PMID: 28968830

Lazaridis K, Dalianoudis I, Baltatzidi V, Tzartos SJ. Specific removal of autoantibodies by extracorporeal immunoadsorption ameliorates experimental autoimmune myasthenia gravis. J Neuroimmunol. 2017 Nov 15;312:24-30. doi: 10.1016/j.jneuroim.2017.09.001. Epub 2017 Sep 6.PMID: 28912035

Altıokka-Uzun G, Erdağ E, Baykan B, Tzartos J, Gezen-Ak D, Samancı B, Dursun E, Zisimopoulou P, Karagiorgou K, Stergiou C, Şahin E, Ekizoğlu E, Kürtüncü M, Tüzün E. Glial and neuronal antibodies in patients with idiopathic intracranial hypertension. Neurol Sci. 2017 Oct;38(10):1817-1822. doi: 10.1007/s10072-017-3066-7. Epub 2017 Jul 21.PMID: 28733756

Cordts I, Bodart N, Hartmann K, Karagiorgou K, Tzartos JS, Mei L, Reimann J, Van Damme P, Rivner MH, Vigneron A, Weis J, Schulz JB, Tzartos SJ, Claeys KG. Screening for lipoprotein receptor-related protein 4-, agrin-, and titin-antibodies and exploring the autoimmune spectrum in myasthenia gravis. J Neurol. 2017 Jun;264(6):1193-1203. doi: 10.1007/s00415-017-8514-z. Epub 2017 May 17.PMID: 28516329

Hong Y, Zisimopoulou P, Trakas N, Karagiorgou K, Stergiou C, Skeie GO, Hao HJ, Gao X, Owe JF, Zhang X, Yue YX, Romi F, Wang Q, Li HF, Gilhus NE, Tzartos SJ. Multiple antibody detection in 'seronegative' myasthenia gravis patients. Eur J Neurol. 2017 Jun;24(6):844-850. doi: 10.1111/ene.13300. Epub 2017 May 4.PMID: 28470860

Kruger JM, Karussis D, Zisimopoulou P, Petrou P. Low-Density Lipoprotein Receptor-Related Protein 4-Positive Myasthenia Gravis in a Double-Seronegative, Electromyography-Negative Patient. J Neuroophthalmol. 2017 Sep;37(3):285-286. doi: 10.1097/WNO.0000000000000499.PMID: 28350570

2016

Lykhmus O, Koval L, Pastuhova D, Zouridakis M, Tzartos S, Komisarenko S, Skok M. The role of carbohydrate component of recombinant α7 nicotinic acetylcholine receptor extracellular domain in its immunogenicity and functional effects of resulting antibodies. 2016 Immunobiology;221(12):1355-1361. doi: 10.1016/j.imbio.2016.07.012.

Kouvatsos N, Giastas P, Chroni-Tzartou D, Poulopoulou C, TzartosSJ. Crystal structure of a human neuronal nAChR extracellular domain in pentameric assembly: Ligand-bound α2 homopentamer. Proc Natl Acad Sci U S A. 2016 Aug 23;113(34):9635-40. doi: 10.1073/pnas.1602619113.

Gilhus NE, Skeie GO, Romi F, Lazaridis K, Zisimoupoulou P, and Tzartos S. Myasthenia gravis-autoantibody characteristics and their implications for therapy. Nat. Rev. Neurol. 2016; 12(5):259-68. doi: 10.1038/nrneurol.2016.44. Epub 2016 Apr 22. Review

Küçükerden M, Huda R, Tüzün E, Yılmaz A, Skriapa L, Trakas N, T. Strait R, Finkelman FD, Kabadayı S, Zisimopoulou P, TzartosS, Christadoss P. MuSK induced experimental autoimmune myasthenia gravis does not require IgG1 antibody to MuSK. J. Neuroimmunol. 2016. Jun 15;295-296:84-92. doi: 10.1016/j.jneuroim.2016.04.003.

Stergiou C, Lazaridis K, Zouvelou V, Tzartos J, Mantegazza R, Antozzi C, Andreetta F, Evoli A, Deymeer F, Saruhan-Direskeneli G, Durmus H, Brenner T, Vaknin A, Berrih-Aknin S, Behin A, Sharshar T, De Baets M, Losen M, Martinez-Martinez P, Kleopa KA, Zamba-Papanicolaou E, Kyriakides T, Kostera-Pruszczyk A, Szczudlik P, Szyluk B, Lavrnic D, Basta I, Peric S, Tallaksen C, Maniaol A, Gilhus NE, Casasnovas Pons C, Pitha J, Jakubíkova M, Hanisch F, Bogomolovas J, Labeit D, Labeit S, TzartosSJ.Titin antibodies in “seronegative” myasthenia gravis – A new role for an old antigen, J. Neuroimmunology, 2016. 292: 108-115. doi: 10.1016/j.jneuroim.2016.01.018.

2015

Lykhmus O, Voytenko L, Koval L, Mykhalskiy S, Cholin V, Peschana K, Zouridakis M, Tzartos S, Komisarenko S and Skok M. α7 Nicotinic Acetylcholine Receptor-Specific Antibody Induces Inflammation And Amyloid β42 Accumulation In The Mouse Brain To Impair Memory. Plos-1, 2015 Mar 27;10(3):e0122706. doi: 10.1371/journal.pone.0122706.

Maurer M, Bougoin S, Feferman T, Frenkian M, Bismuth J, Mouly V, Clairac G, Tzartos S, Fadel E, Eymard B, Fuchs S, Souroujon MC, Berrih-Aknin S. IL-6 and Akt are involved in muscular pathogenesis in myasthenia gravis. Acta Neuropathol Commun. 2015 Jan 15;3(1):1. doi: 10.1186/s40478-014-0179-6.

Azam L, Papakyriakou A, Zouridakis M, Giastas P, Tzartos SJ, McIntosh JM. Molecular Interaction of α-Conotoxin RgIA with the Rat α9α10 Nicotinic Acetylcholine Receptor.Mol Pharmacol. 2015 May;87(5):855-64. doi: 10.1124/mol.114.096511. Epub 2015 Mar 4. PMID:25740413

Arnaouteli S, Giastas P, Andreou A, Tzanodaskalaki M, Aldridge C, Tzartos SJ, Vollmer W, Eliopoulos E, Bouriotis V. Two putative polysaccharide deacetylases are required for osmotic stability and cell shape maintenance in Bacillus anthracis. J Biol Chem. 2015 Mar 30.pii: jbc.M115.640029. [Epub ahead of print] PMID:25825488

Yilmaz V, Oflazer P, Aysa F, Durmus H, Poulas K, Parman Y, Tzartos S, Tuzun E, Deymeer F, Saruhan-Direskeneli G. Differential cytokine changes in patients with myasthenia gravis with antibodies against AChR and MuSK. PLOS-1. 2015; 10(4):e0123546. doi: 10.1371/journal.pone.0123546

Özkök E, Durmus H, Yetimler B, Taslı H, Trakas N, Ulusoy C, Lagoumintzis G, Tzartos S, and Tüzün E: Reduced muscle mitochondrial enzyme activity in MuSK-immunized mice. Clinical Neuropathology. 2015 Nov-Dec;34(6):359-63. doi: 10.5414/NP300875.

Ulusoy C, Zibandeh N, Yıldırım S, Trakas N, Zisimopoulou P, Küçükerden M, Tașlı H, TzartosS, Göker K, Tüzün E, Akkoç T. Dental follicle mesenchymal stem cell administration ameliorates muscle weakness in MuSK-immunized mice. J Neuroinflammation. 2015 Dec 9;12(1):231. doi: 10.1186/s12974-015-0451-0.

Tsonis AI, Zisimopoulou P, Lazaridis K, Tzartos J, Matsigkou E, Zouvelou V, Mantegazza R, Antozzi C, Andreetta F, Evoli A, Deymeer F, Saruhan-Direskeneli G, Durmus H, Brenner T, Vaknin A, Berrih-Aknin S, Behin A, Sharshar T, De Baets M, Losen M, Martinez-Martinez P, Kleopa KA, Zamba-Papanicolaou E, Kyriakides T, Kostera-Pruszczyk A, Szczudlik P, Szyluk B, Lavrnic D, Basta I, Peric S, Tallaksen C, Maniaol A, Casasnovas Pons C, Pitha J, Jakubíkova M, Hanisch F, TzartosSJ. MuSK autoantibodies in myastheniagravis detected by cell based assay-A multinational study. J Neuroimmunol. 2015 Jul 15;284:10-7. doi: 10.1016/j.jneuroim.2015.04.015.

Lykhmus O, Gergalova G, Zouridakis M, Tzartos S, Komisarenko S, Skok M. Inflammation decreases the level of alpha7 nicotinic acetylcholine receptors in the brain mitochondria and makes them more susceptible to apoptosis induction. Int Immunopharmacol. 2015 Nov;29(1):148-51. doi: 10.1016/j.intimp.2015.04.007.

Tuzun E, Berrih-Aknin S, Brenner T, Kusner LL, Le Panse R, Yang H, Tzartos S, Christadoss P. Guidelines for standard preclinical experiments in the mouse model of myasthenia gravis induced by acetylcholine receptor immunization. Exp Neurol. 2015 Feb 16. pii: S0014-4886(15)00032-1. doi: 10.1016/j.expneurol.2015.02.009.

Kusner LL, Losen M, Vincent A, Lindstrom J, Tzartos S, Lazaridis K, Martinez-Martinez P. Guidelines for pre-clinical assessment of the acetylcholine receptor-specific passive transfer myasthenia gravis model-Recommendations for methods and experimental designs. Exp Neurol. 2015 Mar 3.pii: S0014-4886(15)00055-2. doi: 10.1016/j.expneurol.2015.02.025.

Losen M, Martinez-Martinez P, Molenaar PC, Lazaridis K, Tzartos S, Brenner T, Duan RS, Luo J, Lindstrom J, Kusner L. Standardization of the experimental autoimmune myasthenia gravis (EAMG) model by immunization of rats with Torpedo californica acetylcholine receptors – Recommendations for methods and experimental designs. Exp Neurol. 2015 Mar 18.pii: S0014-4886(15)00078-3. doi: 10.1016/j.expneurol.2015.03.010.

2014

Zouvelou V, Zisimopoulou P, Psimenou E, Matsigkou E, Stamboulis E, Tzartos SJ. AChR-myasthenia gravis switching to double-seropositive several years after the onset.J. Neuroimmunol. 2014 Feb 15;267(1-2):111-2. doi: 10.1016/j.jneuroim.2013.12.012.

Tsivgoulis G, Dervenoulas G, Tzartos SJ, Zompola C, Papageorgiou SG, Voumvourakis K. Double seropositive myasthenia gravis with acetylocholine receptor and lipoprotein receptor-related protein 4 antibodies. Muscle and Nerve.2014 Jan 7.doi: 10.1002/mus.24166.

Zis P, Argiriadou V, Temperikidis PP, Zikou L, Tzartos SJ, Tavernarakis A. Parkinson’s disease associated with myasthenia gravis and rheumatoid arthritis. Neurol. Sci.(Letter to the Editor). Published on line 2-7-2014. DOI 10.1007/s10072-014-1660-5

Marino M, Maiuri MT, Di Sante G, Scuderi F, La Carpia F, Trakas N, Provenzano C, Zisimopoulou P, Ria F, Tzartos SJ, Evoli A, Bartoccioni E. T cell repertoire in DQ5-positive MuSK-positive myasthenia gravis patients. J Autoimmun. 2014 Jan 4.pii: S0896-8411(13)00154-6. doi: 10.1016/j.jaut.2013.12.007.

Avidan N, Le Panse R; Harbo H, Bernasconi P, Poulas K, Ginzburg E; Cavalcante P, Colleoni L; Baggi F, Antozzi C, Truffault F, Horn-Saban S, Pöschel S, Zagoriti Z, Maniaol A, Lie B; Bernard I, Saoudi A, Illes Z, Casasnovas C, Melms A, Tzartos S; Willcox N, Kostera-Pruszczyk A, Tallaksen C, Mantegazza R, Berrih-Aknin S, Miller A. VAV1 and BAFF, via NFκB pathway, are genetic risk factors for Myasthenia Gravis. 2014 Annals of Clinical and Translational Neurology,1(5):329-39. doi: 10.1002/acn3.51

Ulusoy C, Kim E, Tüzün E, Huda R, Yılmaz V, Poulas K, Trakas N, Skriapa L, Niarchos A, Strait RT, Finkelman FD, Turan S, Zisimopoulou P, Tzartos S, Saruhan-Direskeneli G, Christadoss P. Preferential production of IgG1, IL-4 and IL-10 in MuSK-immunized mice. Clin Immunol. 2014 Feb 28.pii: S1521-6616(14)00049-7. doi: 10.1016/j.clim.2014.02.012.

Tuzun E., Tzartos J., Ekizoglu E., Stergiou C., Zisimopoulou P., Çoban A., Shugaiv E., Türkoglu R., Kürtüncü M., Baykan B., Tzartos S. Title: Aquaporin-1 Antibody in Neuromyelitis Optica Patients. European Neurology, 2014. Eur Neurol. 2014 Sep 27;72(5-6):271-272.

Kouvatsos N, Niarchos A, Zisimopoulou P, Eliopoulos E, Poulas K, and Tzartos S. Purification and functional characterization of a truncated human α4β2 nicotinic acetylcholine receptor. Int. J Biol Macromol, 2014 Jul 8. pii: S0141-8130(14)00441-3. doi: 10.1016/j.ijbiomac.2014.06.058.

Boltsis I, Lagoumintzis G, Chatzileontiadou DSM, Giastas P, Tzartos SJ, Leonidas DD, and Poulas K. Non-contact Current Transfer Induces the Formation and Improves the X-ray Diffraction Quality of Protein Crystals. 2014 Cryst.Growth Des. DOI: 10.1021/cg5004098

Zouridakis M, Giastas P, Zarkadas E, Chroni-Tzartou D, Bregestovski P and Tzartos SJ. Crystal structures of the free and antagonist-bound states of the extracellular domain of human α9 nicotinic receptor. 2014. Nat. Struct & Mol. Biol. doi:10.1038/nsmb.2900.

Tsivgoulis G, Dervenoulas G, Kokotis P, Zompola C, Tzartos J, Tzartos SJ, Voumvourakis KI. Double seronegative myasthenia gravis with low density lipoprotein-4 (LRP4) antibodies presenting with isolated ocular symptoms. 2014. J. Neurol. Sci. doi: 10.1016/j.jns.2014.09.013.

Kordas G, Lagoumintzis G, Sideris S, Poulas K and Tzartos SJ.Direct proof of the in vivo pathogenic role of the AChR autoantibodies from myasthenia gravis patients.PLOS-1.2014. Sep 26;9(9):e108327. doi: 10.1371/journal.pone.0108327.

Skriapa L, Zisimopoulou P, Trakas N, Grapsa E, Tzartos SJ. Expression of extracellular domains of Muscle Spesific Kinase (MuSK) and use as immunoadsorbents for the development of an antigen specific therapy. 2014. J. Neuroimmunol. DOI: 10.1016/j.jneuroim.2014.09.013

Lazaridis K, Evaggelakou P, Bendenidi E, Sideri A, Grapsa E and Tzartos S.J. Specific adsorbents for myasthenia gravis autoantibodies using mutants of the muscle nicotinic acetylcholine receptor extracellular domains. J. Neuroimmunol. 2015 Jan 15;278:19-25. doi: 10.1016/j.jneuroim.2014.12.001. Kouvatsos N, Niarchos A, Zisimopoulou P, Eliopoulos E, Poulas K, and Tzartos S. Purification and functional characterization of a truncated human α4β2 nicotinic acetylcholine receptor. Int. J Biol Macromol, 2014 Jul 8. pii: S0141-8130(14)00441-3. doi: 10.1016/j.ijbiomac.2014.06.058.

Boltsis I, Lagoumintzis G, Chatzileontiadou DSM, Giastas P, Tzartos SJ, Leonidas DD, and Poulas K. Non-contact Current Transfer Induces the Formation and Improves the X-ray Diffraction Quality of Protein Crystals. 2014 Cryst.Growth Des. DOI: 10.1021/cg5004098

Zouridakis M, Giastas P, Zarkadas E, Chroni-Tzartou D, Bregestovski P and Tzartos SJ. Crystal structures of the free and antagonist-bound states of the extracellular domain of human α9 nicotinic receptor. 2014. Nat. Struct & Mol. Biol. doi:10.1038/nsmb.2900.

Tsivgoulis G, Dervenoulas G, Kokotis P, Zompola C, Tzartos J, Tzartos SJ, Voumvourakis KI. Double seronegative myasthenia gravis with low density lipoprotein-4 (LRP4) antibodies presenting with isolated ocular symptoms. 2014. J. Neurol. Sci. doi: 10.1016/j.jns.2014.09.013.

Kordas G, Lagoumintzis G, Sideris S, Poulas K and Tzartos SJ.Direct proof of the in vivo pathogenic role of the AChR autoantibodies from myasthenia gravis patients.PLOS-1.2014. Sep 26;9(9):e108327. doi: 10.1371/journal.pone.0108327.

Skriapa L, Zisimopoulou P, Trakas N, Grapsa E, Tzartos SJ. Expression of extracellular domains of Muscle Spesific Kinase (MuSK) and use as immunoadsorbents for the development of an antigen specific therapy. 2014. J. Neuroimmunol. DOI: 10.1016/j.jneuroim.2014.09.013

Lazaridis K, Evaggelakou P, Bendenidi E, Sideri A, Grapsa E and Tzartos S.J. Specific adsorbents for myasthenia gravis autoantibodies using mutants of the muscle nicotinic acetylcholine receptor extracellular domains. J. Neuroimmunol. 2015 Jan 15;278:19-25. doi: 10.1016/j.jneuroim.2014.12.001.

2013

Zouvelou V, Stamboulis E, Skriapa L, Tzartos SJ. MuSK-Ab positive myasthenia: Not always grave. J Neurol Sci. 2013, 15;331(1-2):150-1.

Zouvelou V, Kyriazi S, Rentzos M, Belimezi M, Micheli MA, Tzartos SJ, Stamboulis E. Double-seropositive myasthenia gravis. Muscle Nerve. 2013, 47(3):465-6.

Zouvelou V, Zisimopoulou P, Rentzos M, Karandreas N, Evangelakou P, Stamboulis E, Tzartos SJ. Double seronegative myasthenia gravis with anti-LRP 4 antibodies.Neuromuscul Disord. 2013 Jul;23(7):568-70.

Tzartos JS, Stergiou Ch, Kilidireas K, Zisimopoulou P, Thomaidis T, and Tzartos SJ, Aquaporin-1 autoantibodies in patients with neuromyelitis optica spectrum disorders. PLOS-1, 2013.doi: 10.1371/journal.pone.0074773.

Lagoumintzis G, Zisimopoulou P, Trakas N, Grapsas E, Poulas K and Tzartos SJ (2013). Scale up and safety parameters of antigen specific immunoadsorption of human anti-acetylcholine receptor antibodies. J. Neuroimmunol. 2014; 267(1-2):1-6. doi: 10.1016/j.jneuroim. 2013.11.001. Epub 2013 Nov 10.

Zisimopoulou P, Evangelakou P, Tzartos J, Lazaridis K, Zouvelou V, Mantegazza R, Antozzi C, Andreetta F, Evoli A, Deymeer F, Saruhan-Direskeneli G, Durmus H, Brenner T, Vaknin A, Berrih-Aknin S, Frenkian Cuvelier M, Stojkovic T, Debaets M, Losen M, Martinez-Martinez P, Kleopa KA, Zamba-Papanicolaou E, Kyriakides T, Kostera-Pruszczyk A, Szczudlik P, Szyluk B, Lavrnic D, Basta I, Peric S, Tallaksen C, Maniaol A, & Tzartos SJ. A comprehensive analysis of the epidemiology and clinical characteristics of anti-LRP4 in myasthenia gravis. J. Autoimmunity. 2013. Epub ahead of print. http://dx.doi.org/10.1016/j.jaut.2013.12.004

Vrolix K, Fraussen J, Losen M, Stevens J, Lazaridis K, Molenaar PC, Somers V, Bracho MA, Le Panse R, Stinissen P, Berrih-Aknin S, Maessen JG, Van Garsse L, Buurman WA, Tzartos SJ, De Baets MH, Martinez-Martinez P. Clonal heterogeneity of thymic B cells from early-onset myasthenia gravis patients with antibodies against the acetylcholine receptor. J. Autoimmunity. 2014. doi: 10.1016/j.jaut.2013.12.008.

Lazaridis K, Zisimopoulou P, Giastas P, Bitzopoulou K, Evangelakou P; Sideri A, Tzartos SJ. Expression of human AChR extracellular domain mutants with improved characteristics. 2014 J Biol. Macromol;63:210-7. doi: 10.1016/j.ijbiomac.2013.11.003. Epub 2013 Nov 15

Niarchos A, Zouridakis M, Douris V, Georgostathi A, Kalamida D, Sotiriadis A, Poulas K, Iatrou K.. Tzartos SJ. Expression of a highly antigenic and native-like folded extracellular domain of the human α1 subunit of muscle nicotinic acetylcholine receptor, suitable for use in antigen specific therapies for Myasthenia Gravis. 2013 PLOS-1; 8(12):e84791. doi: 10.1371/journal.pone.0084791

Tzartos JS, Zisimopoulou P, Rentzos M, Karandreas N, Zouvelou V, Evangelakou P, Tsonis A, Thomaidis T, Lauria G, Andreetta F, Mantegazza R, Tzartos SJ. LRP4 antibodies in serum and CSF from amyotrophic lateral sclerosis patients. Ann. Clin Transl Neurol. Dec. 2013. doi: 10.1002/acn3.26.

Zisimopoulou P, Brenner T, Trakas N, Tzartos SJ. Serological diagnostics in myasthenia gravis based on novel assays and recently identified antigens. Autoimmun Rev. 2013 Jul;12(9):924-30.

Zagoriti Z, Kambouris M, Patrinos GP, Tzartos S, and Poulas K. (2013). Recent advances in genetic predisposition of myasthenia gravis. BioMed Res Int. Epub 2013 Nov 5.

2012

Zagoriti Z, Georgitsi M, Giannakopoulou O, Ntellos F, Tzartos SJ, Patrinos GP, Poulas K. Genetics of myasthenia gravis: a case-control association study in the Hellenic population. Clin Dev Immunol. 2012;2012:484919. doi:10.1155/2012/484919. Epub 2012 Sep 25.

Poulas K, Koutsouraki E, Kordas G, Kokla A, Tzartos SJ. Anti-MuSK- and anti-AChR-positive myasthenia gravis induced by d-penicillamine. J Neuroimmunol. 2012 Sep 15;250(1-2):94-8. doi: 10.1016/j.jneuroim.2012.05.011.

Tzartos, J., C. Stergiou, H. Alexopoulos, P. Zisimopoulou, C. Karageorgiou, K. Kilintireas, M. Dalakas, and S. Tzartos, (2012) Highly Sensitive Radioimmunoassay Identifies Anti-Aquaporin-4 Autoantibodies in Several “Seronegative” Patients Suspected for Neuromyelitis Optica-Spectrum Disorders (NMO). Neurology, 78, P02133.

Zhang, B., J.S. Tzartos, M. Belimezi, S. Ragheb, B. Bealmear, R.A. Lewis, W.C. Xiong, R.P. Lisak, S.J. Tzartos, and L. Mei, (2012) Autoantibodies to Lipoprotein-Related Protein 4 in Patients With Double-Seronegative Myasthenia Gravis. Archives of Neurology. 69(4): p. 445-451.

2011

Trakas, N., P. Zisimopoulou, and S.J. Tzartos, Development of a highly sensitive diagnostic assay for muscle-specific tyrosine kinase (MuSK) autoantibodies in myasthenia gravis. 2011 Journal of Neuroimmunology. 240: p. 79-86.

Koval, L., O. Lykhmus, O. Kalashnyk, N. Bachinskaya, G. Kravtsova, M. Soldatkina, M. Zouridakis, C. Stergiou, S. Tzartos, V. Tsetlin, S. Komisarenko, and M. Skok, (2011) The Presence and Origin of Autoantibodies Against alpha 4 and alpha 7 Nicotinic Acetylcholine Receptors in the Human Blood: Possible Relevance to Alzheimer’s Pathology. Journal of Alzheimers Disease. 25(4): p. 747-761.

Lykhmus, O., L. Koval, M. Skok, M. Zouridakis, P. Zisimopoulou, S. Tzartos, V. Tsetlin, S. Granon, J.P. Changeux, S. Komisarenko, and I. Cloez-Tayarani, (2011) Antibodies against Extracellular Domains of alpha4 and alpha7 Subunits Alter the Levels of Nicotinic Receptors in the Mouse Brain and Affect Memory: Possible Relevance to Alzheimer’s Pathology. Journal of Alzheimers Disease. 24(4): p. 693-704.

Pavlakis, P.P., H. Alexopoulos, M.L. Kosmidis, E. Stamboulis, J.G. Routsias, S.J. Tzartos, A.G. Tzioufas, H.M. Moutsopoulos, and M.C. Dalakas, (2011) Peripheral neuropathies in Sjogren syndrome: a new reappraisal. Journal of Neurology Neurosurgery and Psychiatry. 82(7): p. 798-802.

Stergiou, C., P. Zisimopoulou, and S.J. Tzartos, (2011) Expression of Water-soluble, Ligand-binding Concatameric Extracellular Domains of the Human Neuronal Nicotinic Receptor alpha 4 and beta 2 Subunits in the Yeast Pichia pastoris; glycosylation is not required for ligand binding. Journal of Biological Chemistry. 286(11): p. 8884-8892.

2010

Keefe, D., C. Parng, D. Lundberg, S. Ray, J. Martineau-Bosco, C. Leng, S. Tzartos, J. Powell, M. Concino, M. Heartlein, J. Lamsa, and S. Josiah, (2010) In vitro characterization of an acetylcholine receptor-transferrin fusion protein for the treatment of myasthenia gravis. Autoimmunity. 43(8): p. 628-639.

Lagoumintzis, G., P. Zisimopoulou, G. Kordas, K. Lazaridis, K. Poulas, and S.J. Tzartos, (2010) Recent approaches to the development of antigen-specific immunotherapies for myasthenia gravis. Autoimmunity. 43(5-6): p. 436-445.

Gattenlohner, S., H. Jorissen, M. Huhn, A. Vincent, D. Beeson, S. Tzartos, A. Mamalaki, B. Etschmann, H.K. Muller-Hermelink, E. Koscielniak, S. Barth, and A. Marx, A Human Recombinant Autoantibody-Based Immunotoxin Specific for the Fetal Acetylcholine Receptor Inhibits Rhabdomyosarcoma Growth In Vitro and in a Murine Transplantation Model. Journal of Biomedicine and Biotechnology Art. No. 187621.

2009

Keefe, D., Hess, D., Dosco, J., Tzartos, S., Powell, J., Lamsa. J., and Josiah, S. (2009) A rapid, fluorescence based assay for detecting antigenic modulation of the acetylcholine receptor on human cell lines. Cytometry B Clin Cytom. 76(3):206-12.

Zouridakis M, Zisimopoulou P, Eliopoulos E, Poulas K, and Tzartos SJ (2009) Design and expression of human α7 nicotinic acetylcholine receptor extracellular domain mutants with enhanced solubility and ligand-binding properties. Bioch. Bioph. Acta. 1794: 355-66.

Tsiamalos, P., Kordas, G., Kokla, A., Poulas, K., and Tzartos, S.J. (2009). Epidemiological and immunological profile of MuSK myasthenia gravis in Greece. Eur. J. Neurol. 16: 925-30.

Lykhmus, O , Koval, L., Pavlovych, S., Zouridakis, M., Zisimopoulou, P., Tzartos, S., Tsetlin, V., Volpina, O., Cloëz-Tayarani, I., Komisarenko, S., and Skok, M. (2009) Functional effects of antibodies against non-neuronal nicotinic acetylcholine receptors. Immunol. Lett. 128(1):68-73.

Gattenlohner, S, Jörißen, H., Huhn, M., Vincent, A., Beeson, D., Tzartos, S., Mamalaki, A., Etschmann, B., Müller-Hermelink, H.K., Koscielniak, E., Barth S.and Marx, A. (2009). A human recombinant autoantibody-based immunotoxin specific for the fetal acetylcholine receptor inhibits rhabdomyosarcoma growth in vitro and in a murine transplantation model. J. Biomed. Biotech. 2010:187621. doi: 10.1155/2010/187621

Keefe, D., Parng , C., Lundberg, D., Ray, S., Martineau-Bosc, J., Leng, C., Tzartos, S., Powell , J., Concino, M., Michael Heartlein, M., Lamsa. J., and Josiah, S. (2010). In vitro characterization of an acetylcholine receptor-transferrin fusion protein for the treatment of Myasthenia Gravis. Autoimmunity, 43(8):628-39

2008

Konstantakaki, M, Tzartos, S.J., Poulas, K. and Eliopoulos, E. (2008). Μodel of the extracellular domain of the human α7 nAChR based on the crystal structure of the mouse α1 nAChR extracellular domain. J. Mol. Graph Modelling. 26: 1333-7.

Bitzopoulou, K., ,Kostelidou, K., Poulas, K and Tzartos, S.J. (2008) Expression and characterization of mutant forms of the extracellular domain of the human AChR gamma-subunit with improved solubility and enhanced antigenicity. Biochem. Biophys. Acta. 1784: 1226-33.

Zisimopoulou, P., Lagoumintzis, G., Poulas, K. and Tzartos, S.J. (2008). Antigen-specific apheresis of human anti-acetylcholine receptor autoantibodies from myasthenia gravis patients’ sera using Escherichia coli-expressed receptor domains. J. Neuroimmmunol. 200: 133-141.

Yi, H.J., Chae, C.S., So, J.S., Tzartos, S.J. Souroujon MC, Fuchs, S., Im, SH (2008) Suppression of experimental myasthenia gravis by a B-cell epitope-free recombinant acetylcholine receptor. Mol. Immunol. 461:192-201.

2007

Konstantakaki, M., Tzartos, S.J., Poulas, K., and Eliopoulos, E. (2007) Molecular modeling of the complex between Torpedo acetylcholine receptor and anti-MIR Fab198. Biochem. Biophys. Res. Commun. 356:569-75.

Sideris, S., Lagoumintzis, G., Kordas, G., Kostelidou, K., Sotiriadis, A, Poulas, K. and Tzartos, S.J. (2007) Isolation and functional characterization of anti-acetylcholine receptor subunit-specific autoantibodies from myasthenic patients: receptor loss in cell culture. J. Neuroimmunol. 189:111-7.

Zouridakis, M., Kostelidou, K., Sotiriadis, A., Stergiou, Ch. Eliopoulos, E., Poulas, K., and Tzartos, S. (2007) Circular Dichroism Studies of Recombinant Extracellular Domains of Human Muscle and Neuronal Nicotinic Acetylcholine Receptors Provide an Insight into their structure. Intl. J. Biol. Macromol. 41:423-9.

Kostelidou, K., Trakas,N. and Tzartos, S.J. (2007) Extracellular domains of the β, γ and ε subunits of the human acetylcholine receptor as immunoadsorbents for myasthenic autoantibodies: a combination of immunoadsorbents results in increased efficiency. J. Neuroimmmunol. 190:44-52

2006

Kostelidou, K., Trakas,N., Zouridakis, M., Bitzopoulou, K., Sotiriadis,A., Gavra, H., and Tzartos, S.J. (2006). Expression and characterization of soluble forms of the extracellular domains of the β, γ and ε subunits of the human muscle acetylcholine receptor. FEBS J. 273: 3557-3568

Fostieri, E., Tzartos, S.J., Berrih-Aknin, S., Beeson, D. and Mamalaki A. (2005) Isolation of potent human Fab fragments against a novel highly immunogenic region on human muscle acetylcholine receptor, which protect the receptor from myasthenic autoantibodies. Eur. J. Immunol. 35:632-643.

2000-2005

Protopapadakis, E., Kokla, A., Tzartos, S.J. and Mamalaki, A (2005) Isolation and characterization of human anti-acetylcholine receptor monoclonal antibodies from transgenic mice expressing human immunoglobulin loci. Eur. J. Immunol. 35:1960-68.

Guyon, S., Christadoss, P., Lepanse, R, Guyon, T, DePoea Baets, M., Wakkach, A., Bidault, J., Tzartos, S., and Berrih-Aknin, S. (2005) Effects of cytokines on AChR expression. Implications for myasthenia gravis. J. Immunol. 174: 5941-49.

Psaridi-Linardaki, L., Mamalaki, A., Trakas, N. and Tzartos, S.J. (2005) Specific immunoadsorption of the autoantibodies from myasthenic patients using the extracellular domain of the human muscle acetylcholine receptor α-subunit. Development of an antigen-specific therapeutic strategy. J. Neuroimmunol. 159:183-91.

Avramopoulou, V., Mamalaki, A., Tzartos, S.J. (2004) Soluble, oligomeric and ligand-binding extracellular domain of human alpha7 acetylcholine receptor expressed in yeast. Replacement of the hydrophobic cys-loop by the hydrophilic loop of ACh-binding protein enhances protein solubility. J. Biol. Chem. 279: 38287-93.

Marinou, M. and Tzartos, S.J. (2003). Identification of regions involved in the binding of α-Bungarotoxin to the human α7 neuronal nicotinic acetylcholine receptor using synthetic peptides. Biochem. J. 372, 543-554.

Phan-Chan-Du, A., Hemmerlin, C., Krikorian, D., Sakarellos-Daitsiotis, M., Tsikaris, V., Sakarellos, C., Marinou. M.,Thureau, A., Cung, M.T. and Tzartos, S.J. (2003). Solution conformation of the antibody-bound tyrosine phosphorylation site of the nicotinic acetylcholine -subunit in its phosphorylated and nonphosphorylated states. Biochemistry, 42:7371-7380.

Campos, E.C., Schiavi, C., Bolognesi, A., Bellusci, C., Lubelli, C., Duca, A., Polito, L., Vismara, S., Poulas, K., Tzartos, S.I., and Stirpe, F. (2002). Selective lesions of rabbit extraocular muscles injected with the anti-AChR immunotoxin saporin-mAb 73. Curr. Eye Res. 24: 58-65.

Psaridi-Linardaki, L.,Mamalaki, A., Remoundos, M. and Tzartos, S.J. (2002). Expression of soluble ligand- and antibody-binding extracellular subunit in yeast Pichiadomain of human muscle acetylcholine receptor α- pastoris. Role of glycosylation in α-bungarotoxin binding. J. Biol. Chem. 277: 26980-6.

Μetaxas, A., Tzartos, S., Liakopoulou-Kyriakide, M. (2002). The production of anti-hexapeptide antibodies which recognize the S7, L6 and L13 ribosomal proteins of Escherichia coli. J Pept Sci. 8:118-24.

Tataridis, D., Kolocouris, A., Fytas, G., Kolocouris, N., Foscolos, G.B., Poulas, K. and Tzartos, S.J. (2002).Mounting the nicotinic pharmacophoric structural elements in an homoadamantane scaffold: synthesis, molecular modeling and binding affinities to α7 nicotinic acetylcholine receptors. IL Farmaco. 57:979-984.

Poulas, K. and Tzartos, S.J (2001). The gender gap in autoimmune disease. Letter to Lancet, 357, 234.

Poulas, K., Eliopoulos, E., Vatzaki, E., Navaza, J., Kontou, M., Oikonomakos, N., Acharya, K.R., and Tzartos, S. J. (2001). Crystal structure of Fab198, an efficient protector of acetylcholine receptor against myasthenogenic antibodies. Eur. J. Biochem. 268: 3685-3693.

Poulas, K., Tsibri, E., Papanastasiou, D., Tsouloufis, T., Marinou, M., Tsantili, P., Papapetropoulos, T., and Tzartos, S.J. (2001). Epidemiology of seropositive myasthenia gravis in Greece. J. Neurol. Neurosur. Psych. 71:352-6.

Theodorou, V.,Tsikaris, V., Sakarellos-Daitsiotis, M, Avramopoulou, V., Kostelidou, K., Tzartos, S.J. and Sakarellos, C. (2001). Design, synthesis and conformational study of biologically active photolabeled analogues of the Main Immunogenic Region of the acetylcholine receptor. Biopolymers. 56: 37-46.

Trakas, N. and Tzartos, S.J. (2001) Conjugation of acetylcholine receptor-protecting Fab fragments with polyethylene glycol results in a prolonged half-life in the circulation and reduced immunogenicity. J. Neuroimmunol. 120:42-49.

Skok, M., Lykhmus, E., Bobrovnik, S., Tzartos, S.I., Tsouloufis, T., Vanderesse, R. , Coutrot, F., Cung, M.T., Marraud, M., Krikorian, D., and Sakarellos-Daitsiotis, M. (2001). Structure of epitopes recognized by the antibodies to alpha (181-192) peptides of neuronal nicotinic acetylcholine receptors: extrapolation to the structure of acetylcholine-binding domain. J. Neuroimmunol. 121: 59-66.

Kleinjung, J., Petit, M-C., Orlewski, P., Mamalaki, A., Tzartos, S.J., Tsikaris, V., Sakarellos-Daitsiotis, M., Sakarellos, C., Marraud, M. and Cung. M.-T. (2000). The 3D structure of the complex between an Fv antibody fragment and an analogue of the main immunogenic region of the acetylcholine receptor: A combined 2D-NMR, homology and molecular modeling approach. Biopolymers. 53: 113-128

Sevin-Landais, A., Rigler, P., Tzartos, S., Hucho, F., Hovius, R. and Vogel, H. (2000). Immobilisation of the nicotinic acetylcholine receptor in tethered lipid membranes. Biophys. Chem. 85:141-52.

Poulas, K., Tsouloufis, Th., and Tzartos S.J. (2000) Treatment of passively transferred experimental autoimmune myasthenia gravis using papain. Clin. Expl. Immunol. 120:363-368.

Tsouloufis, T., Mamalaki, A., Remoundos, M. and Tzartos, S.J. (2000). Reconstitution of conformationally-dependent epitopes on the N-terminal extracellular domain of the human muscle acetylcholine receptor α-subunit expressed in E. coli. Implications for Myasthenia Gravis therapeutic approaches. Int. Immunol. 12: 1255-65.

Papanastasiou, D., Poulas, K., Kokla, A and Tzartos, S. J. (2000). Prevention of passively transferred experimental autoimmune myasthenia gravis by Fab fragments of monoclonal antibodies directed against the main immunogenic region of the acetylcholine receptor. J. Neuroimmunol. 104:124-132.

Kontou, M., Leonidas, D., Vatzaki, E.H., Tsantili, P., Mamalaki, A., Oikonomakos, N.G., Acharya, K.R, and Tzartos, S. J. (2000).The crystal structure of the Fab fragment of a rat monoclonal antibody against the main immunogenic region of the human muscle acetylcholine receptor. Eur. J. Biochem. 267:2389-2397

Michalet, S., Teixeira, F., Gilquin, B., Mourier, G., Servent, S., Drevet, P., Binder, P., Tzartos, S., Ménez, A., and Kessler, P. (2000). Relative spatial position of a snake neurotoxin and the reduced disulfide bond αCys192-Cys193) at the αγ interface of the nicotinic acetylcholine receptor. J. Biol. Chem. 275: 25608-25615.

Sieb, J.P., Kraner, S., Schrank, B., Reitter, B., Goebel, H.H., Tzartos, S. J. and Steinlein, O.K. (2000). Severe congenital myasthenic syndrome due to homozygosity of the 1293insG ε-AChR subunit mutation. Ann. Neurol. 48:379-83

Fostieri, E., Beeson, D. and Tzartos, S.J. (2000). The conformation of the main immunogenic region on the α-subunit of muscle acetylcholine receptor is affected by neighboring receptor subunits. FEBS Lett. 481: 127-130.

Poulas, K., Tsibri, E., Papanastasiou, D., Tsouloufis, T., Marinou, M., Tsantili, P., Papapetropoulos, T., and Tzartos, S.J. (2000). Equal male and female incidence of myasthenia gravis. Neurology, 54, 1202-1203.

1990-1999

Nenninger, R., Schultz, A., Helmreich, M., Wilisch, A., Vandekerckhove, B., Hunig, T., Schalke, B., Tzartos, S.J., Kahlbacher, H., Muller-Hermelink, H. and Marx, A. (1998). Abnormal thymocyte development – Generation of autoaggressive T-cells in mixed and cortical thymomas. Lab. Invest. 78:743-753.

Tsantili, P., Tzartos, S.J. and Mamalaki, A. (1999) High affinity scFv antibody fragments protecting the human nicotinic acetylcholine receptor. J. Neuroimmunol. 94: 15-27.

Papanastasiou, D., Mamalaki, A., Eliopoulos, E., Poulas, K., Liolitsas Ch. and Tzartos, S.J. (1999). Construction and characterization of a humanized single chain Fv antibody fragment against the main immunogenic region of the acetylcholine receptor. J. Neuroimmunol. 94: 182-195.

Tzartos, S.J. and Remoundos, M. (1999). Detection of antibodies directed against the cytoplasmic region of the human acetylcholine receptor in sera from myasthenia gravis patients. Clin. Expl. Immunol. 116: 146-152.

Skok, M.V., Voitenko, L. P., Voitenko, S. V., Lykhmus, E. Yu., Kalashnik, E. N., Litvin, T. I., Tzartos S. J. and Skok, V. I. (1999). Alpha subunit composition of nicotinic acetylcholine receptors in the rat autonomic ganglia neurons as determined with subunit-specific anti-α(181-192) peptide antibodies. Neuroscience. .93: 1436-1447.

Wilisch, A., Gutsche, S., Hoffacker, V., Schultz, A., Tzartos, S., Nix, W., Schalke, B., Schneider, C., Muller-Hermelink, H. K. and Marx A. (1999). Association of acetylcholine receptor α-subunit expression in mixed thymoma with myasthenia gravis. Neurology. 52: 1460-1466.

Schultz, A., Hoffacker, V.,Wilisch, A., Nix, W., Schalke, B., Nix, W., Tzartos, S., Muller-Hermelink, H. K. and Marx A. (1999). Neurofilament is an autoantigenic determinant in myasthenia gravis. Ann. Neurology. 46: 167-75.

Martin-Ruiz, C.M., Court, J.A., Molnar, Ε., Lee, Μ., Gotti, C., Mamalaki, A., Tsouloufis, T., Tzartos, S., Ballard, C., Perry, R.H. and Perry, E.K. (1999) Alpha 4 but not alpha 3 and 7 nicotinic acetylcholine receptor subunits are lost from the temporal cortex in Alzheimer’s disease. J. Neurochem. 73: 1635-1640.

Jacobson, L., Beeson, D., Tzartos, S. and Vincent, A. (1999). Monoclonal antibodies raised against human acetylcholine receptor bind to all five subunits of the fetal isoform. J. Neuroimmunol. 98: 112-120.

Wakkach, A., Poea, S., Chastre, E., Gespach, C., Lecerf, F., De la Porte, S., Tzartos, S., Coulombe, A., and Berrih-Aknin, S. (1999). Establishment of a thymic myoid cell line: phenotypic and functional characteristics. Am. J. Pathol. 155:1229-1240

Sieb, J.P., Dorfler, P., Tzartos, S., Wewer, U., Ruegg, M.,Meyer, D., Baumann, I., Lindemuth, R., Jakschik, J. and Ries, F. (1998). Congenital myasthenic syndromes in two kinships with endplate acetylcholine receptor and utrophin deficiency. Neurology, 50: 54-61.

De la Porte, S., Chaubourt, E., Fabre, F., Poulas, K., Chapron, J., Eymard, B., Tzartos, S.J., and Koenig, J. (1998). Accumulation of acetylcholine receptors is a necessary condition for normal accumulation of acetylcholinesterase during in vivo neuromuscular synaptogenesis. Eur. J. Neurosci., 10: 1631-1643.

Barchan, D., Asher, O., Tzartos, S.J., Fuchs, S. and Souroujon, M. (1998). Modulation of the anti-acetylcholine receptor and experimental autoimmune myasthenia gravis by recombinant fragments of the acetylcholine receptor. Eur. J. Immunol. 28:616-624.

Gattenloehner, S., Vincent, A., Leuschner, I., Tzartos, S., Kirchner, T., Muller-Hermelink, H.-K., Marx, A. (1998) Investigation of the acetylcholine receptor in rhabdomyosarcomas as compared with other childhood tumors. Am. J. Pathol. 152: 437-444.

Guyon, T., Wakkach, A., Klingel-Schmitt, I., Levasseur, P., Beeson, D., Asher, O., Tzartos, S.J. and Berrih-Aknin, S. (1998). Regulation of acetylcholine receptor gene expression in human myasthenia gravis muscles. Evidence for a compensatory mechanism triggered by receptor loss. J. Clin. Invest. 102: 249-263.

Orlewski, P., Marraud, M., Cung, M.T, Tsikaris, V., Sakarellos-Daitsiotis, M., Sakarellos, K., Vatzaki, E. and Tzartos, S.J. (1996). Compared structures of the free AChR MIR decapeptide and the antibody-bound [A76]MIR analogue. A molecular dynamics simulation from 2D-NMR data. Biopolymers (Peptide Science). 40: 419-432.

Gotti, C., Balestra, B., Mantegazza, R., Tzartos, S., Moretti, M. & Clementi, F. (1997) Detection of antibody subpopulations in myasthenia gravis using a non radioactive new enzyme immunoassay. Muscle & Nerve, 20: 800-808.

Loutrari, H., Kokla, A., N. Trakas and Tzartos, S.J. (1997). Expression of human-Torpedo hybrid acetylcholine receptors for analyzing the antigenic specificities in myasthenic antisera. Clin. Expl. Immunol. 109, 538-546.

Andre, F., Marraud, M., Tsouloufis, T., Tzartos, S.J., Boussard, G. (1997) Triphosgene: An efficient carbonylating agent for liquid and solid-phase aza-peptide synthesis. Application to the synthesis of two aza-analogues of the AChR MIR decapeptide. J. Pep. Science . 3: 429-441

Voltz, R., Kamm, C., Padberg, F., Malotka, J., Kerschensteiner, M., Spuler, S., Tzartos, S., and Dornmair, K. (1997) Highly purified oligo-His tagged human recombinant alpha(1)-AChR is immunogenic in vivo and suitable for T cell stimulation in vitro in experimental and human myasthenia gravis. J.Neuroimmunol. 80:131-136.

Bufler, J., Kahlert, S., Tzartos, S.J., Toyka, C. and Franke, C. (1996). Activation and blockage of nicotinic channels by antibodies directed against the binding site of the acetylcholine receptor. J. Physiol. 492: 107-114

Kontou, M., Vatzaki, E.H., Kokla, A., Acharya, K.R., Oikonomakos, N.G. and Tzartos, S.J. (1996). Characterisation, crystallisation and preliminary X-ray diffraction analysis of a Fab fragment of a rat monoclonal antibody with very high affinity for the human muscle acetylcholine receptor. FEBS Lett. 389: 195-198.

Wakkach, A., Guyon, T., Bruand, C., Tzartos, S., Cohen-Kaminsky, S. and Berrih-Aknin, S. (1996) Expression of acetylcholine receptor genes in human thymic epithelial cells. Implication for myasthenia gravis. J. Immunol. 157: 3752-3760.

Pappas, I.S., Sophianos, D., Tzartos, S.J. and Tsiftsoglou, A.S. (1996). Expression of memory, differentiation and repression of c-myc and p53 genes in human RD/TE-671 cells induced by a uveido-derivative of pyridine (UDP-4). Cell Growth and Differentiation. 7: 797-809.

Tsikaris, V., Sakarellos, C., Sakarellos-Daitsiotis, M., Orlewski, P., Marraud, M. Cung, M.T., Vatzaki, E., and Tzartos, S.J. (1996). Construction and application of a new class of sequential oligopeptide carriers for multiple anchoring of antigenic peptides. Application to the AChR main immunogenic region. Intern. J. Biol. Macromol. 19: 195-205.

Tzartos, S.J., Kouvatsou, R. and Tzartos E. (1995) Monoclonal antibodies as site-specific probes for the acetylcholine receptor δ-subunit tyrosine and serine phosphorylation sites. Eur. J. Biochem. 228, 463-472.

Mapouras, D., Philippou, G., Charalabous, S., Tzartos, S.J. Balafas, A., Souvatzoglou, A. and Lymberi, P. (1995). Antibodies to acetylcholinesterase cross-reacting with thyroglobulin in myasthenia gravis and graves disease. Clin. Exp. Immunol 100: 336-343.

Tzartos, S.J., Tzartos, E., and Tzartos, J.S. (1995) Monoclonal antibodies against the acetylcholine receptor γ-subunit as site specific probes for receptor tyrosine phosphorylation. FEBS Letters. 363: 195-198.

Mamalaki, A., Boutou, E., Hurel, C., Patsavoudi, E., Tzartos, S.J. and Matsas, R. (1995). The BM88 antigen, a novel neuron-specific molecule, promotes the differentiation of mouse neuroblastoma cells. J. Biol. Chem. 270: 14201-14208.

Asher, O., Kues, W.A., Witzemann, V.,Tzartos, S.J., Fuchs, S. and Souroujon, M.C. (1993) Increased gene expression of acetylcholine receptor and myogenic factors in passively transferred experimental autoimmune myasthenia gravis. J. Immunol. 151, 6442-6450.

Schmutz, M., Kling, D., Tzartos, S.J. and Brisson, A. (1994). A mixed ELISA-immunoelectron microscopy approach for studying epitope topology of membrane proteins: application to the acetylcholine receptor. J. Histochem. Cytochem. 42, 315-327 (1994).

Tzartos, S.J., Valcana, C., Kouvatsou, R., and Kokla, A. (1993) The tyrosine phosphorylation site of the acetylcholine receptor β-subunit is located in a highly immunogenic epitope implicated in channel function. Antibody-probes for β subunit phosphorylation and function. EMBO J. 12, 5141-5149.

Papadouli, I., Sakarellos, C.,and Tzartos, S.T. (1993). High resolution epitope mapping and fine antigenic characterization of the main immunogenic region of the acetylcholine receptor. Improving the binding activity of synthetic analogues of the region. Eur. J. Biochem. 211, 227-234.

Eleftheriou, C.S., Trakas, N., Kokla, A. and Tzartos, S.J. (1993). A group of three fibroblast secreted polypeptides suppressed by cellular ageing and interferon-γ. Bioch. Bioph. Acta. 1180, 304-312.

Detsikas, E., Tsikaris, V., Sakarellos-Daitsiotis, M., Sakarellos, C., Cung, M.T., Marraud, M., Vatzaki, E. and Tzartos, S.J. (1993). Cyclic lactam analogues containing the main immunogenic region of Torpedo acetylcholine receptor. Peptide Research 6, 17-23.

Tsikaris, V., Detsikas, E., Sakarellos-Daitsiotis, M., Sakarellos, C., Vatzaki, E., Tzartos, S.J., Marraud, M. and Cung, M.T., (1993). Conformational requirements for molecular recognition of ACh receptor MIR analogues by monoclonal anti-MIR antibody: A 2D-NMR and molecular dynamics approach. Biopolymers. 33, 1123-1134.

Mamalaki, A., Trakas, N., and Tzartos, S.J. (1993). Bacterial expression of a single-chain Fv fragment which efficiently protects the acetylcholine receptor against antigenic modulation caused by myasthenic antibodies. Eur. J. Immunol. 23, 1839-1845.

Melms, A., Weissert, R., Klinkert, W.E.F., Schalke, B.C.G., Tzartos, S. and Wekerle, H. (1993). Specific immune complexes augment in vitro acetylcholine receptor-specific T-cell proliferation. Neurology 43, 583-588.

Vatzaki, E. H., Acharya, K.R., Oikonomakos, N. G. and Tzartos, S.J. (1993). Crystallization and preliminary crystallographic study of an Fab fragment of a pathogenic rat monoclonal antibody against the nicotinic acetylcholine receptor. Protein Science. 2: 1770-1772.

Loutrari, H., Tzartos, S.J. and Claudio, T. (1992). Use of Torpedo-mouse hybrid acetylcholine receptors reveals immunodominance of the α-subunit in myasthenia gravis antisera. Eur. J. Immunol. 22, 2949-2956.

Loutrari, H., Kokla, A. and Tzartos, S.J. (1992). Passive transfer of experimental myasthenia gravis via antigenic modulation of acetylcholine receptor. Eur. J. Immunol. 22, 2449-2452.

Zhang, Y., and Tzartos, S.J. (1992). B-T lymphocyte interactions in experimental autoimmune myasthenia gravis. Autoantibody mediated up-regulation of the response of AChR-specific T-lymphocytes. Immunology 77, 571-576.

Marx, A., Osborn, M., Tzartos, S., Geuder, K.I., Schalke, B., Nix, W., Kirchner, T., and Muller-Hermelink, H.K. (1992). A striational muscle antigen and myasthenia gravis-associated thymomas share an acetylcholine receptor epitope. Develop. Immunol. 2, 77-84.

Cung, M.T., Demange, P., Marraud, M., Tsikaris, V., Sakarellos, C., Papadouli, I., Kokla, A., Tzartos, S.J. (1991). Two-dimentional 1H-NMR study of antigen-antibody interactions: binding of synthetic decapeptides to an anti-acetylcholine receptor monoclonal antibody. Biopolymers. 31, 769-776.

Verschuuren, J.J.G.M., Graus Y.M.F., Bos N.A., Tzartos S.J., Van Breda Vriesman P.J.C., and De Baets M.H. (1991). Paratope and framework related idiotopes on acetylcholine receptor antibodies. J. Immunol. 146, 941-948.

Verschuuren, J.J.G.M., Graus Y.M.F., Van Breda Vriesman P.J.C., Tzartos S.J., and De Baets M.H. (1991). In vivo effects of neonatal administration of anti-idiotype antibodies on experimental autoimmune myasthenia gravis Autoimmunity. 10:173-179.

Tzartos, S. J., Loutrari, H., Tang, F., Kokla, A., Walgrave, S., Milius, R. P. and Conti-Tronconi, B. (1990). Main immunogenic region of Torpedo electroplax and human muscle acetylcholine receptor. Localization and micro-heterogeneity reveiled by the use of synthetic peptides. J. Neurochem. 54, 51-61.

Plinkert, P.K., Gitter, A.H., Zimmermann, U., Kirchner, T., Tzartos, S. and Zenner, H.P. (1990). Visualization and functional testing of acetylcholine receptor-like molecules in cochlear outer hair cells. Hearing Res. 44, 25-34.

Marx, A., O’ Connor, R., Geuder, K.I., Hoppe, F., Schalke, B., Tzartos, S., Kalies, I., Kirchner, T., and Muller-Hermelink, H. K. (1990). Characterization of a protein with an acetylcholine receptor epitope from myasthenia gravis-associated thymomas. Lab. Invest.. 62, 279-286.

Tzartos, S.J., Efthimiadis, A., Morel, E. and Bach, J.F. (1990) Neonatal myasthenia gravis: antigenic specificities of antibodies in sera from mothers and their infants. Clin. Exp. Immunol. 80, 376-380.

Papadouli, I., Potamianos, S., Hadjidakis, I., Bairaktari, H., Tsikaris, V., Sakarellos, C., Cung, M.T., Marraud, M. and Tzartos, S.J. (1990). Antigenic role of single residues within the main immunogenic region of the nicotinic acetylcholine receptor. Biochem. J. 269, 239-245.

Tzartos, S.J and Remoundos, M.S. (1990). Fine localization of the major α-bungarotoxin binding site to residues α189-195 of the Torpedo acetylcholine receptor. Residues 189, 190 and 195 are indispensable for binding. J. Biol. Chem. 265, 21462-21467.